全文获取类型
收费全文 | 1953篇 |
免费 | 127篇 |
国内免费 | 62篇 |
专业分类
儿科学 | 12篇 |
妇产科学 | 1篇 |
基础医学 | 262篇 |
口腔科学 | 20篇 |
临床医学 | 91篇 |
内科学 | 110篇 |
皮肤病学 | 4篇 |
神经病学 | 274篇 |
特种医学 | 64篇 |
外科学 | 260篇 |
综合类 | 275篇 |
预防医学 | 133篇 |
眼科学 | 13篇 |
药学 | 438篇 |
2篇 | |
中国医学 | 166篇 |
肿瘤学 | 17篇 |
出版年
2024年 | 3篇 |
2023年 | 18篇 |
2022年 | 28篇 |
2021年 | 76篇 |
2020年 | 53篇 |
2019年 | 44篇 |
2018年 | 42篇 |
2017年 | 52篇 |
2016年 | 60篇 |
2015年 | 48篇 |
2014年 | 100篇 |
2013年 | 91篇 |
2012年 | 105篇 |
2011年 | 119篇 |
2010年 | 101篇 |
2009年 | 92篇 |
2008年 | 86篇 |
2007年 | 105篇 |
2006年 | 106篇 |
2005年 | 80篇 |
2004年 | 61篇 |
2003年 | 51篇 |
2002年 | 41篇 |
2001年 | 30篇 |
2000年 | 38篇 |
1999年 | 32篇 |
1998年 | 37篇 |
1997年 | 36篇 |
1996年 | 23篇 |
1995年 | 28篇 |
1994年 | 27篇 |
1993年 | 29篇 |
1992年 | 24篇 |
1991年 | 27篇 |
1990年 | 22篇 |
1989年 | 26篇 |
1988年 | 28篇 |
1987年 | 19篇 |
1986年 | 19篇 |
1985年 | 34篇 |
1984年 | 21篇 |
1983年 | 18篇 |
1982年 | 19篇 |
1981年 | 13篇 |
1980年 | 10篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1970年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有2142条查询结果,搜索用时 15 毫秒
1.
【目的】 了解学术期刊专题/专栏建设的现状,以切实发挥学术期刊专题/专栏建设的作用,为期刊的品牌建设、学术创新、影响力提升和办刊质量提高提供策略。【方法】 采用问卷调查法,对全国范围内397名专题/专栏合作对象(包括期刊主编、编委、审稿人、作者)进行调查,了解他们对期刊专题/专栏建设的态度和意见,从合作对象视角探讨影响学术期刊专题/专栏建设成功率和有效性的因素。【结果】 合作对象态度上,表示支持专题/专栏建设工作的比例较高(71.28%),但供稿率不高(43.37%),存在态度和行为分离的现象。排名前5的供稿率影响因素是期刊被本专业领域研究者认可、期刊被数据库收录情况、期刊影响力、期刊品牌、期刊的处理速度。最优的约稿方式是行业内的学术带头人联系约稿,其次是熟识的专家委托和期刊主编亲自联系进行约稿。合作对象最希望获得的帮助是稿件快速审理发表和确定选题方向。【结论】 为提高学术期刊专题/专栏建设质量,应进一步挖掘重要合作对象的作用,有针对性地选择高供稿率的合作对象;努力挖掘和利用影响合作对象供稿的因素,在期刊品牌建设、扩大影响力方面下功夫;优化约稿方式方法,重视行业学术带头人的作用;同时通过稿件快速审理发表、帮助确定选题并提供发表后推广等服务,提高合作对象对专题/专栏建设工作的认可度和参与度。 相似文献
2.
《Transfusion Clinique et Biologique》2022,29(1):94-97
Dynamic monitoring ABO chimera including erythroid ABO antigen and anti-A/B is crucial to not only assess the status of erythroid engraftment but also achieve personalized safety transfusion in patients post ABO incompatible hematopoietic stem cell transplantation. Transfusion support for ABO incompatible (ABOi) HSCT patients after achieved complete alteration to donor origin still remains cautious because the instant hematopoietic status on these transplant patients possibly returned to patient origin derived from early disease relapse and graft loss or failure. We reported that reemergent anti-B in a female patients (donor/patient: B/O) at the early phase after achievement complete donor type were not effectively found from partial automatic ABO blood grouping systems, which directly resulted in differential judgement of transplantation stage for about 15 days and disturbed the optimal recommendation on transfusion support. Meanwhile, the solely alteration of ABO chimera was found and earlier than changes of other markers such as MRD diagnosis, chimerism analysis by STR-PCR and sex chromosome assays, which can be an available predictors for bad transplant outcomes such as graft failure. 相似文献
3.
目的 制备高纯度3,5-O-二咖啡酰基奎宁酸,并评价其对人宫颈癌HeLa细胞的抗增殖活性。方法 采用柱色谱提取法和中压液相色谱法从奇蒿花中分离、纯化得到高纯度的3,5-O-二咖啡酰基奎宁酸。采用MTT法评价该化合物对人宫颈癌HeLa细胞的体外抗增殖活性。结果 柱色谱提取的提取率和中压液相色谱法的回收率分别为99.0%,61.2%,总回收率为54.0%。随着3,5-O-二咖啡酰基奎宁酸浓度升高,HeLa细胞存活率下降,细胞形态损伤增加,受试药物的IC50值为26.5µg·mL-1。结论 本研究提供了一种简单、高效、节能的3,5-O-二咖啡酰基奎宁酸制备方法。3,5-O-二咖啡酰基奎宁酸对HeLa细胞具有一定的体外抗增殖活性。 相似文献
4.
Aneta Kholov Ivona Lhotsk Adla Uhrov Ivan pnik Andrea Machykov Petr Solich Frantiek vec Dalibor atínský 《Toxins》2020,12(12)
According to the EU legislation, ochratoxin A contamination is controlled in wines. Tokaj wine is a special type of sweet wine produced from botrytized grapes infected by “noble rot” Botrytis cinerea. Although a high contamination was reported in sweet wines and noble rot grapes could be susceptible to coinfection with other fungi, including ochratoxigenic species, no screening of Tokaj wines for mycotoxin contamination has been carried out so far. Therefore, we developed an analytical method for the determination of ochratoxin A (OTA) and ochratoxin B (OTB) involving online SPE coupled to HPLC-FD using column switching to achieve the fast and sensitive control of mycotoxin contamination. The method was validated with recoveries ranging from 91.6% to 99.1% with an RSD less than 2%. The limits of quantification were 0.1 and 0.2 µg L−1 for OTA and OTB, respectively. The total analysis time of the online SPE-HPLC-FD method was a mere 6 min. This high throughput enables routine analysis. Finally, we carried out an extensive investigation of the ochratoxin contamination in 59 Slovak Tokaj wines of 1959–2017 vintage. Only a few positives were detected. The OTA content in most of the checked wines did not exceed the EU maximum tolerable limit of 2 µg L−1, indicating a good quality of winegrowing and storing. 相似文献
5.
6.
7.
8.
Antonio Montalvo Afonso Fernando Ruiz Juretschke Rosario González Rodrigálvarez Olga Mateo Sierra Begoña Iza Vallejo Roberto García Leal Marc Valera Melé Vicente Casitas Hernando Jose Manuel Hernández Poveda Carlos Fernández Carballal 《Neurocirugía (Asturias, Spain)》2021,32(1):1-9
Background and objectivesThe treatment of deafferentation pain by spinal DREZotomy is a proven therapeutic option in the literature. In recent years, use of DREZotomy has been relegated to second place due to the emergence of neuromodulation therapies. The objectives of this study are to demonstrate that DREZotomy continues to be an effective and safe treatment and to analyse predictive factors for success.Patients and methodsA retrospective study was conducted of all patients treated in our department with spinal DREZotomy from 1998 to 2018. Bulbar DREZotomy procedures were excluded. A visual analogue scale (VAS) and the reduction of routine medication were used as outcome variables. Demographic, clinical and operative variables were analysed as predictive factors for success.ResultsA total of 27 patients (51.9% female) with a mean age of 53.7 years underwent DREZotomy. The main cause of pain was brachial plexus injury (BPI) (55.6%) followed by neoplasms (18.5%). The mean time of pain evolution was 8.4 years with a mean intensity of 8.7 according to the VAS, even though 63% of the patients had previously received neurostimulation therapy. Favourable outcome (≥ 50% pain reduction in the VAS) was observed in 77.8% of patients during the postoperative period and remained in 59.3% of patients after 22 months average follow-up (mean reduction of 4.9 points). This allowed for a reduction in routine analgesic treatment in 70.4% of them. DREZotomy in BPI-related pain presented a significantly higher success rate (93%) than the other pathologies (41.7%) (p = .001). No association was observed between outcome and age, gender, DREZ technique, duration of pain or previous neurostimulation therapies. There were six neurological complications, four post-operative transient neurological deficits and two permanent deficits.ConclusionDorsal root entry zone surgery is effective and safe for treating patients with deafferentation pain, especially after brachial plexus injury. It can be considered an alternative treatment after failed neurostimulation techniques for pain control. However, its indication should be considered as the first therapeutic option after medical therapy failure due to its good long-term results. 相似文献
9.
We have established a novel hydrophilic chromatography (HILIC)-high performance liquid chromatography (HPLC) method to assess sialic acid content in food products. Single-factor and response surface methodologies (RSM) were used to systematically optimize the hydrolysis conditions of the food samples to extract the maximum amount of sialic acid. Chromatographic conditions were also adjusted. In foods containing sialic acid, we observed a strong linear relationship between sialic acid and peak area, ranging from 5 to 100 μg/mL (R2 = 0.9998). The lowest detectable sialic acid concentration (RSN = 3) was 0.2 μg mL−1, and the method detection limit was 0.02mg kg−1. Sample recovery ranged from 95.85% to 99.78%, with an RSD of 1.46% (n = 6). Thus, the described method can be applied to the study of sialic acid content in foods. 相似文献
10.
Mengyuan Wang Christopher M. Stafford Lewis M. Cox Adrienne K. Blevins Masoud Aghajani Jason P. Killgore Yifu Ding 《Macromolecular chemistry and physics.》2019,220(13)
Controlled growth of crosslinked polyamide (PA) thin films is demonstrated at the interface of a monomer‐soaked hydrogel and an organic solution of the complementary monomer. Termed gel–liquid interfacial polymerization (GLIP), the resulting PA films are measured to be chemically and mechanically analogous to the active layer in thin film composite (TFC) membranes. PA thin films are prepared using the GLIP process on both a morphologically homogeneous hydrogel prepared from poly(2‐hydroxyethylmethacrylate) and a phase‐separated, heterogeneous hydrogel prepared from poly(acrylamide). Two monomer systems are examined: trimesoyl chloride (TMC) reacting with m‐phenylene diamine (MPD) and TMC reacting with piperazine (PIP). Unlike the self‐limiting growth behavior in TFC membrane fabrication, diffusion‐limited, continuous growth of the PA films is observed, where both the thickness and roughness of the PA layers increase with reaction time. A key morphological difference is found between the two monomer systems using the GLIP process; TMC/MPD produces a ridge‐and‐valley surface morphology whereas TMC/PIP produces nodule/granular structures. The GLIP process represents a unique opportunity to not only explore the pore characteristics (size, spacing, and continuity) on the resulting structure and morphology of the interfacially polymerized thin films, but also a method to modify the surface of (or encapsulate) hydrogels. 相似文献